Effect of process parameters on the antioxidant activities of bioactive compounds from Harad (Terminalia chebula retz.) Shilpa Soni, H.K. Sharma*, Pragati Kaushal and C. Singh Food Engineering & Technology Department, Sant Longowal Institute of Engineeri

Abstract

Terminalia chebula retz (T.Chebula) is a herb known as king of medicinal plants in ayurvedic world. Presence of large number of phytoconstituents makes T.Chebula a potential source of nutraceuticals and can become cynosure of modern nutraceutical world. The objective of this study was to optimize the extraction conditions for bioactive constituents from T.Chebula fruits using Response Surface Methodology (RSM). A central composite face centered design (CCD) was employed. The extraction conditions for bioactive constituents from T. Chebula fruits were optimized by using three independent process variables i.e. methanol concentration, 50-90%, extraction temperature, 50-70°C and extraction time, 30-60 min. Effect of extraction parameters was found to be significant. The optimum extraction conditions were identified as 90% methanol concentration, 70°C temperature and 59.94 minutes for maximum total phenolic content(TPC), 3.87 GAE mg/g, total flavonoid content (TFC), 361.37 CE mg/g, total antioxidant activity(TAA), 0.158 AAE mM/g and α,α-diphenyl-β-picrylhydrazyl (DPPH) scavenging activity (DSA), 86.28 %. Experimental values for response variables under the optimum conditions were found reasonably close to the predicted value. GC–MS analysis of methanol extract of T.Chebula fruits under the optimum conditions led to the identification of 7 major bioactive compounds.

Keywords: T.Chebula, Extraction, Total Phenolic compound (TPC), Total flavonoid compound (TFC), Total antioxidant activity (TAA), α,α-diphenyl-β-picrylhydrazyl (DPPH) scavenging activity (DSA), Response surface methodology (RSM), GC-MS.