Evaluation, Identification and Characterization of Micro - Organisms in Raw and Processed Orange Fruits using different Techniques


  • SUNMONU MUSLIU OLUSHOLA department of food engineering, university of ilorin, nigeria
  • ODEWOLE MICHAEL MAYOKUN university of ilorin
  • SANUSI MAYOWA SAHEED University of Ilorin
  • AJALA OLAWALE ELIJAH university of ilorin
  • OYEDEJI HENRY FOLAHAN university of ilorin


orange, juice, coliform, plate, count.


In this study, different samples of Orange fruits/juice were analyzed using Pour Plate Method (PPM) and Spread Plate Method (SPM). Samples analyzed includes; Raw orange Fruit (RF) serving as control, Manually Extracted orange Juice (MEJ), Machine Extracted orange Juice (MAEJ), Pasteurized Machine Extracted orange Juice at 850C (PMAEJ, 850C) and Refrigerated Machine Extracted orange Juice at 40C (RMAEJ, 40C). The objective of the experiment was to evaluate the micro-organisms count in the raw and processed orange fruits using the PPM and the SPM .  The micro-organisms count considered include; Total Heterotrophic Plate Count (THPC), Total Coliform Count (TCC), Total Faecal Coliform Count (TFCC), and Fungal Count (FC). A total of 15 micro-organisms were identified which were Aeromonas hydrophila, Bacillus subtilis, Micrococcus sp., Staphylococcus aureus, Staphylococcus epidermis, Listeria sp., Lactobacillus fermenti, Providencia sp., Klebsiella pneumonia, Enterobacter aerogenes, Sacchoronyees cereoisioe, Penicillium sp. Aspergillus niger, Rhizopus stolonifer, and  Nucor racemosus.

Author Biographies

SUNMONU MUSLIU OLUSHOLA, department of food engineering, university of ilorin, nigeria

department of food engineering/ Senior Lecturer

ODEWOLE MICHAEL MAYOKUN, university of ilorin

department of food engineering/ lecturer 1

SANUSI MAYOWA SAHEED, University of Ilorin

food engineering/lecturer II

AJALA OLAWALE ELIJAH, university of ilorin

department of chemical engineering/Senior Lecturer

OYEDEJI HENRY FOLAHAN, university of ilorin

department of agricultural and biosystems engineering






VI-Postharvest Technology and Process Engineering