Optimization of the pulsed electric field -assisted extraction of functional compounds from Nepeta binaludensis

Afsaneh Azimi Mahalleh, Parvin Sharayei, Seyed Ali Mortazavi, Elham Azarpazhooh, Razieh Niazmand


Pulsed electric field (PEF) treatment was used for extracting effective components from Nepeta (Nepeta binaludensis Jamzad). A response surface method was used to investigate the effects of independent process variables (voltage of pulsed electric field (VPEF): 2, 4 and 6 KV/cm and number of pulsed electric field (NPEF): 20, 40 and 60 n) on the yield (Y) and antioxidant characteristics: total phenolic compounds (TPC), 1,1- diphenyl -2- picrylhydrazyl free radical scavenging (DPPHsc), ferric reducing-antioxidant power (FRAP), half  maximal of radical-scavenging activity (IC50) of Nepeta extract (aerial parts). According to Derringer’s desired function approach, the optimal conditions based on both individual and combinations of all process variables were VPEF 6KV/cm and NPEF 60 n. At this optimum condition, the Y, TPC, DPPHSC, FRAP, and IC50 of the extract were found to be 11.36%, 417.85 mg GA/g, 74.8%, 1688.53µmol Fe2+/g, and 0.32 mg/mL, respectively. The experimental values were in a good agreement with the predicted values. Also, the extract at optimal conditions of PEF (PEFopt-x) had a higher quantity of chlorogenic acid, caffeic acid, rutin, para-coumaric acid, rosemarinic acid, Kaempferol, and apigenin compared with solvent extract. The addition of PEFopt-x to the purified soybean oil at the levels of 6% increased oxidative stability index (2.65 h) close to butylated hydroxy toluene (2.78h).


Phenolic compounds; Nepeta binaludensis; Response Surface Methodology; Pulsed electric field; Antimicrobial activity

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