In vitro Photobiological Studies on Two Woody Tree species

Abstract

The enforcement work was accomplishments in vitro (Tissue culture and Germplasm Conservation Research Lab.) to study the effect of different laser rays in inducing growth behavior and leaf anatomical structure on Balanites aegyptiaca and  Contoneoster horizontalis,.

     Exposing Planlets of Balanites plants developing in vitro to various types of lasers (helium-neon laser, cadmium neon laser and Argon neon laser) at 20 minutes exposure time each separately. The results showed increase in vegetative growth performance, photosynthetic pigments and anatomical characteristics. Exposure time 20 min. He-Ne were more effective it increased of root length, root number, chlorophyll b, carotenoids, the thickness of both lamina and midvine, the number of xylem rows and number of vessels and dimensions bundle.While,20 min. exposure time Cd-Ne recorded highest values in shoot length, leaves number, chlorophyll a  and leaf anatomical structure as compared to untreated plants .

        Exposing Planlets of Cotoneoster horizontalis plant in vitro to helium-neon laser, at 0, 2.5, 6.5 and 11.5 mints exposure time. The results showed increase in vegetative growth, photosynthetic pigments and anatomical characteristics. Treated plantlets with 2.5 min. exposure time He-Ne recorded highest increasing in shoot length, chlorophyll a, b, the thickness of lamina and midvine, the number of xylem rows, number of vessels and palisade thickness. 11.5 min. exposure time recorded increasing in leaves number, root length, root number, dimensions bundle and spongy thickness as compared to plantlets control plants.

Key words: in vitro, laser rays, woody trees, leaf anatomy, photobiology