Assessment of biospeckle activity of lemon fruit

Abstract

Bio-speckle can be used as a method for analysing the activity of biological materials illuminated with laser beam.  Physically, bio-speckles are the result of scattering of coherent light on moving particles inside living tissue.  Therefore, the aim of this study was to investigate bio-speckle activity in lemons and determine the age of the lemon fruit from the observation of its dynamic speckle pattern.  The speckle pattern of laser light scattered in lemon fruits were measured through their quantification.  For the quantification of the variation by bio-speckle, two different methods of image analysis were used: moment of inertia and the spatial temporal speckle correlation coefficient.  The bio-speckle activities were also characterized by the line profiles of time history speckle pattern (THSP) which contains data of time information of dynamic speckle.  Furthermore, measure of the dynamic speckle varies for fruits as their quality decrease, and the values change with the position where the images are taken.