Application of visible and near-infrared spectrophotometry for detecting salinity effects on wheat leaves (Triticumasativum L.)

Mohammad Hossein Mokhtari, Hamid Reza Sodae, Mohammad Ali Hakimzadeh, Fahimeh Tarighat


Soil and water salinity is the most limiting factor for plant growth and productivity. Due to a high rate of evaporation, agricultural lands become saline in arid regions after a while. This leads to a decline in plant production. The present study investigated the capability of visible and near infrared (VNIR) spectrophotometry as a non-destructive method in detecting salinity effect on wheat leaves. A completely randomized design was work out with four salinity levels and three replicates. Wheat seeds were planted in plastic pots and irrigated with four levels of saline water [0 (control), 4, 8 and 12 dSm-1] Leaf spectrophotometry at VNIR (190-1100 nm) wavelength was performed on wheat leaves at the nodule-formation growth stage. The results indicated that treatments are discriminated mostly by reflectance and absorption spectra of 530-660 nm although a difference existed between the control treatment and the other treatments at 700-1100 nm. The difference between the treatments of T0, T4 and T12 was found to be significant (P<0.01) in the reflectance with an absorption value of 530-660. Although all the treatments are discriminated at 700-1100nm visually, the difference between them was statistically insignificant at this wave range.


Salinity, Wheat, Spectroscopy, Visible and Near-infrared

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